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Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth
Edition is a must-have collection of methods and procedures on how
to create a single, continuous, comprehensive project that teaches
students basic molecular techniques. It is an indispensable tool
for introducing advanced undergraduates and beginning graduate
students to the techniques of recombinant DNA technology-or gene
cloning and expression. The techniques used in basic research and
biotechnology laboratories are covered in detail. Students will
gain hands-on experience on subcloning a gene into an expression
vector straight through to the purification of the recombinant
protein.
This manual is an indispensable tool for introducing advanced
undergraduates and beginning graduate students to the techniques of
recombinant DNA technology, or gene cloning and expression. The
techniques used in basic research and biotechnology laboratories
are covered in detail. Students gain hands-on experience from start
to finish in subcloning a gene into an expression vector, through
purification of the recombinant protein.
The second edition has been completely re-written, with new
laboratory exercises and all new illustrations and text, designed
for a typical 15-week semester, rather than a 4-week intensive
course. The project approach to experiments was maintained:
students still follow a cloning project through to completion,
culminating in the purification of recombinant protein. It takes
advantage of the enhanced green fluorescent protein-students can
actually visualize positive clones following IPTG induction.
*Cover basic concepts and techniques used in molecular biology
research labs
*Student-tested labs proven successful in a real classroom
laboratories
*Exercises simulate a cloning project that would be performed in a
real research lab
*"Project" approach to experiments gives students an overview of
the entire process
*Prep-list appendix contains necessary recipes and catalog numbers,
providing staff with detailed instructions"
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